Aptamer Details

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TTA1.2 (ID# 8052)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Tenascin-C (TN-C)

Antigen/Target Category: Protein

Affinity (Kd): 2.4 nM (reported value)

Binding Conditions/Buffer: TBSMC buffer (20 mM Tris, pH 7.4; 137 mM NaCl; 1 mM CaCl2; 1 mM MgCl2)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Aptamer was modified post selection from a 2'F library. Note that there is an internal PEG4 linker between G9 and C10. This linker stabilizes the secondary structure and is critical for binding.

Sequence ():

Length: 38

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Schmidt KS, Borkowski S, Kurreck J, Stephens AW, Bald R, Hecht M, et al. Application of locked nucleic acids to improve aptamer in vivo stability and targeting function. Nucleic Acids Res 2004; 32:5757- 65; PMID:15509871; http://dx.doi.org/10.1093/nar/ gkh862

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