Aptamer Details

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S21 (ID# 8065)

Aptamer Chemistry: DNA

Target: Cas9

Antigen/Target Category: Protein

Affinity (Kd): 0.63 nM (reported value)

Binding Conditions/Buffer: 20 mM HEPES, pH 6.5, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 47

Molecular Weight: 14334.3

Extinction Coefficient: 418100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.

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