Aptamer Details

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CTBA4T-B1 (ID# 8088)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Peptide

Affinity (Kd): 19 pM (reported value)

Binding Conditions/Buffer: Binding buffer (1× PBSMT, pH 7.2) containing 137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.76 mM KH2PO4, 1 mM MgCl2, and 0.025% Tween-20.

Binding Temp: 95, 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Letters in yellow are nucleotides in the original central constant domain. Letters in blue are nucleotides in the two random-sequence regions. Those in red are nucleotides in the primer domains.

Sequence ():

Length: 55

Molecular Weight: 17110.1

Extinction Coefficient: 525200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Mao, Yu et al. “Evolution of a highly functional circular DNA aptamer in serum.” Nucleic acids research vol. 48,19 (2020): 10680-10690. doi:10.1093/nar/gkaa800

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