Aptamer Details

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AP32-4 (ID# 8118)

Aptamer Chemistry: DNA

Target: Metronidazole

Antigen/Target Category: Small Organic

Affinity (Kd): 77.22 ± 11.27 nM (reported value)

Binding Conditions/Buffer: 200mL binding buffer: 50 mM Tris-HCl containing 5 mM KCl, 100 mM NaCl and 1 mMMgCl2, pH 7.4

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 16

Molecular Weight: 4639

Extinction Coefficient: 143000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wei, Hao et al. “Screening and application of a truncated aptamer for high-sensitive fluorescent detection of metronidazole.” Analytica chimica acta vol. 1128 (2020): 203-210. doi:10.1016/j.aca.2020.07.003

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