Aptamer Details

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CBSA-5335 SF (ID# 8120)

Aptamer Chemistry: DNA

Target: Cocaine

Antigen/Target Category: Small Organic

Affinity (Kd): 36 nM (reported value)

Binding Conditions/Buffer: 10% saliva in 10 mM Tris, 0.1 mM MgCl2, pH 7.4

Binding Temp: 23°C

Refolding Protocol: N/A

Specificity: Demonstrated specificity to cocaine over benzoylecgonine, anhydroecgonine methyl ester, cocaethylene and nicotine

Comments: The sequence below is one fragment of the cocaine split aptamer that contains two separate fragments, please view "Related Aptamers" for second fragment of the sequence. "X" in sequence stands for C3 Spacer

Sequence ():

Length: 19

Molecular Weight: 5793.8

Extinction Coefficient: 198200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yu, H., Canoura, J., Guntupalli, B., Lou, X., & Xiao, Y. (2017). A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva. Chemical Science, 8(1), 131–141. doi:10.1039/c6sc01833e

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