Aptamer Details

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B01 (ID# 8128)

Aptamer Chemistry: DNA

Target: β2-Microglobulin (B2M)

Antigen/Target Category: Protein

Affinity (Kd): 52.9 nM (reported value)

Binding Conditions/Buffer: Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 80

Molecular Weight: 24550.8

Extinction Coefficient: 732200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ma, Y.; Li, W.; Xing, R.; Li, P.; Liu, Z. Epitope-Imprinted Magnetic Nanoparticles as a General Platform for Efficient In Vitro Evolution of Protein-Binding Aptamers. ACS Sens. 2020 Jul 7; 5(8): 2537-2544. DOI: 10.1012/acssensors.0c00846

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