Aptamer Details

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S6-1b (ID# 8136)

Aptamer Chemistry: DNA

Target: Membrane protein of glioma cell line SHG44

Antigen/Target Category: Protein

Affinity (Kd): 3.1-44 nM (reported value)

Binding Conditions/Buffer: 4.5 g/L glucose, 0.01 M PBS, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 53

Molecular Weight: 16291.6

Extinction Coefficient: 513000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lin, N.; Wu, L.; Xu, X.; Wu, Q.; Wang, Y.; Shen, H.; Song, Y.; Wang, H., Zhu, Z.; Kang, D.; Yang, C. Aptamer Generated by Cell-SELEX for Specific Targeting of Human Glioma Cells. ACS Appl. Mater. Interfaces. 2021 Mar 3; 13(8): 9306-9315. DOI: 10.1021/acsami.0c11878.

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