Aptamer Details

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A10 (ID# 8138)

Aptamer Chemistry: DNA

Target: Bovine pregnancy-associated glycoproteins (bPAGs)

Antigen/Target Category: Protein

Affinity (Kd): 11.7 nM (reported value)

Binding Conditions/Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4

Binding Temp: 94°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 56

Molecular Weight: 17849.5

Extinction Coefficient: 545500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Liu, C., Lu, C., & Shi, G. (2020). Selection, identification, and application of DNA aptamers against bovine pregnancy-associated glycoproteins 4. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-020-02666-w

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