Aptamer Details

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f-4 (ID# 8155)

Aptamer Chemistry: DNA

Target: fetuin

Antigen/Target Category: Other

Affinity (Kd): 6.2 µM (reported value)

Binding Conditions/Buffer: 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20, 20 mM Tris-HCl, pH 7.5

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Contains indole-modified bases. See reference article and supplement for details.

Sequence ():

Length: 40

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms Alex M. Yoshikawa, Alexandra Rangel, Trevor Feagin, Elizabeth M. Chun, Leighton Wan, Anping Li, Leonhard Moekl, Michael Eisenstein, Sharon Pitteri, H. Tom Soh bioRxiv 2021.03.13.435263; doi: https://doi.org/10.1101/2021.03.13.435263

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