Aptamer Details

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h-Ras (ID# 8184)

Aptamer Chemistry: DNA

Target: h-Ras protein

Antigen/Target Category: Protein

Affinity (Kd): 200 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris-HCl at pH 8.2

Binding Temp: 20°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: N/A

Sequence ():

Length: 80

Molecular Weight: 24507.9

Extinction Coefficient: 741700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Berezovski, M., Musheev. M., Drabovich, A., and S. N. Krylov. 2006. Non-SELEX Selection of Aptamers. Journal of the American Chemical Society. 128, 1410-1411. doi: 0.1021/ja056943j

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