Aptagen, LLC
Clon-3 (ID# 8193)
DNA
PD-L1
Protein
70.1 ± 14.2 nM nM (reported value)
Binding buffer:
50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES),
100 mM NaCl, 1 mM MgCl2, 5 mM KCl, and 1 mM CaCl2, pH =
7.5
Binding/Wash buffer (Capture SELEX):
10 mM Tris, 1 mM EDTA,
1 M NaCl, pH 7.4
Varying incubation times:
10min - 1hr
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
N/A
5'-dCpdCpdCpdTpdCpdCpdTpdCpdCpdTpdApdApdCpdTpdGpdTpdTpdCpdCpdTpdApdCpdGpdApdApdApdCpdGpdApdGpdCpdTpdTpdApdTpdGpdCpdGpdTpdApdApdTpdGpdApdTpdGpdApdCpdTpdGpdTpdCpdGpdTpdApdGpdTpdTpdCpdGp-3'
60
18462
640.4
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Li, J., Ren, X., Zhao, J., & Lou, X. (2021). PD-L1 aptamer isolation via Modular-SELEX and its applications in cancer cell detection and tumor tissue section imaging. The Analyst, 146(9), 2910–2918. https://doi.org/10.1039/d1an00182e
Have your aptamer oligo synthesized ORDER
We are always looking for ways to improve. Please tell us what you think.