Aptamer Details

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BB16-11 f (ID# 8210)

Aptamer Chemistry: DNA

Target: Bifidobacterium Breve

Antigen/Target Category: Cells

Affinity (Kd): 18.66 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris–HCl (pH 7.4), 100 mM NaCl, 5 mM KCl and 1 mM MgCl2.

Binding Temp: 21°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: N/A

Comments: N/A

Sequence ():

Length: 29

Molecular Weight: 8972.73

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Hu, L., Wang, L., Lu, W., Zhai, Q., Fan, D., Liu, X., … Chen, W. (2017). Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breve. RSC Advances, 7(19), 11672–11679.

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