Aptagen, LLC
MBMI-01c (ID# 8222)
DNA
α-conotoxin MI (CTX-MI)
Peptide
0.524 nM (reported value)
The selection Buffer (SB) (0.9 mM CaCl2, 2.7 mM KCl, 1.5 mM KH2PO4,
0.6 mM MgCl2·6H2O, 0.1 M NaCl and 20 mM Na2HPO4, pH = 7.4) was purchased from
Tiandz (Beijing, China). The SB was used for aptamer screening, the BLI assay, and the
performance evaluation of the aptasensor.
20°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specificity detection was conducted along with CTX-GI, domoic acid, okadoic acid, saxitoxin, and tetrodoxin. High response values with no significant difference were monitored when CTX-MI or the mixture of the aforesaid six toxins were added, while there was almost no response in other groups, suggesting that MBMI-01c was bound to CTX-MI with specific high affinity.
5'-dApdTpdTpdGpdGpdCpdApdCpdTpdCpdCpdApdCpdGpdCpdApdTpdApdGpdGpdTpdTpdTpdGpdGpdGpdGpdApdTpdGpdGpdGpdCpdApdApdCpdGpdGpdTpdApdApdApdApdApdGpdGpdGpdTpdCpdApdApdApdApdGpdGpdCpdTpdTpdTpdTpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
80
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Guo, H.; Deng, B.; Zhao, L.; Gao, Y.; Zhang, X.; Yang, C.; Zou, B.; Chen, H.; Sun, M.; Wang, L.; et al. Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI. Toxins 2022, 14, 706. https://doi.org/10.3390/toxins14100706
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