Aptamer Details

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HD1-60.29-15dA (ID# 8223)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): 4.9 nM (reported value)

Binding Conditions/Buffer: 1x PBS, 3mM Magnesium Chloride. 1 ug/uL BSA.

Binding Temp: 20°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Paper suggests this aptamer as an improvement on the classic thrombin binding aptamer

Sequence ():

Length: 59

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Müller J, Wulffen B, Pötzsch B, Mayer G. Multidomain targeting generates a high-affinity thrombin-inhibiting bivalent aptamer. Chembiochem. 2007 Dec 17;8(18):2223-6. doi: 10.1002/cbic.200700535. PMID: 17990265.

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