Aptagen, LLC
S1 Sars-CoV-2 Apt-126 (ID# 8446)
DNA
S1 Protein from SARS-CoV-2
Protein
0.8 nM (reported value)
Aptamer can work in either Buffer 1 or Buffer 2. Buffer 1 contained 25 mM Tris pH 8.0, 150 mM NaCl. Buffer 2 contained 10 mM HEPES pH 7, 150 mM NaCl, 3 mM EDTA, 0.05% Tween 20, 0.5 mg/mL BSA, 0.05 mg/mL salmon sperm DNA.
24 °C
Heated at 90°C for 3 minutes and then cooled to 4°C for 5 minutes. BLI: BLI binding buffer (10 mM HEPES pH 7, 150 mM NaCl, 3 mM EDTA, 0.05% Tween 20) by heating to 95°C for 5 minutes and then cooling to 4°C for 5 minutes.
This Aptamer is characterized as a TNA aptamer, this means that the sugar back bone uses threose nucleic acids instead of deoxyribose nucleic acids. Instead of the normal 3' to 5' bonds there are a 3' to 2' bonds.
5'-dGpdCpdCpdApdTpdGpdTpdTpdGpdTpdTpdGpdGpdTpdCpdGpdGpdGpdApdTpdGpdGpdTpdGpdApdApdCpdGpdCpdTpdCpdCpdGpdTpdGpdTpdTpdGpdCpdGp-3'
40
12422
371000
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lozoya-Colinas, A., Yu, Y., & Chaput, J. C. (2023b). Functionally enhanced XNA aptamers discovered by parallelized library screening. Journal of the American Chemical Society, 145(47), 25789–25796. https://doi.org/10.1021/jacs.3c09497
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