Aptagen, LLC
SNAP1 Aptamer SARS-CoV-2 S Protein (ID# 8565)
DNA
SARS-CoV-2 Spike Protein
Protein
60.4 nM (reported value)
4.5 g/L glucose, 0.1 g/L CaCl2, 0.2 g/L KCl, 0.2 g/L KH2PO4, 0.1 g/L
MgCl2·6H2O, 8 g/L NaCl, 2.1716 g/L Na2HPO4, and 5 mM MgCl2, 1% BSA, 0.1 mg/mL tRNA, 0.1 mg/mL SS DNA, 0.01% Tween-20 @ 25 °C
74.4 ºC°C
N/A
Thiol-labeled gold nanoparticle-conjugated version used as the Reporter Aptamer in a Sandwich Lateral Flow Assay with a detection limit of 100 pM Spike Protein (6 x 10^9 copies), and a detection limit of 10^6 copies/mL of virus.
5'-dTpdCpdGpdCpdTpdCpdTpdTpdTpdCpdCpdGpdCpdTpdTpdCpdTpdTpdCpdGpdCpdGpdGpdTpdCpdApdTpdTpdGpdTpdGpdCpdApdTpdCpdCpdTpdGpdApdCpdTpdGpdApdCpdCpdCpdTpdApdApdGpdGpdTpdGpdCpdGpdApdApdCpdApdTpdCpdGpdCpdCpdCpdGpdCpdGpdTpdApdApdGpdTpdCpdCpdGpdTpdGpdTpdGpdTpdGpdCpdGpdApdAp-3'
86 nt
26361 g/mole
779500 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lucy F. Yang, Nataly Kacherovsky, Nuttada Panpradist, Ruixuan Wan, Joey Liang, Bo Zhang, Stephen J. Salipante, Barry R. Lutz, and Suzie H. Pun. Aptamer sandwich lateral flow assay (AptaFlow) for antibody-free SARS-CoV-2 detection. Analytical Chemistry 2022 94 (20), 7278-7285. DOI: 10.1021/acs.analchem.2c00554
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