Aptamer Details

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Benzodiazepine (20DF42 Aptamer) (ID# 8580)

Aptamer Chemistry: DNA

Target: Benzodiazepine

Antigen/Target Category: Small Organic

IC50: 1.01 µM (reported value)

Binding Conditions/Buffer: Annealing Buffer: 50 mM Tris pH 8.0 150 mM NaCl Wash Buffer: Supplemental Urine (1x Formula) Sodium sulfate Na2SO4 5.98 mM Uric acid (stock in 1 M NaOH – Add equal amount of 1 M HCl to SU to neutralize excess NaOH) C5H4N4O3 0.74 mM Trisodium citrate dihydrate Na3C6H5O7•2H2O 1.23 mM Creatinine C4H7N3O 3.90 mM Urea CH4N2O 124.88 mM Potassium chloride KCl 15.48 mM Sodium chloride NaCl 105.50 mM Calcium chloride dihydrate CaCl2•2H2O 0.83 mM Ammonium chloride NH4Cl 11.83 mM Potassium oxalate monohydrate K2C2O4•H2O 0.10 mM Magnesium sulfate heptahydrate MgSO4•7H2O 2.19 mM Sodium phosphate monobasic dihydrate NaH2PO4•H2O 9.33 mM Sodium phosphate dibasic dihydrate Na2HPO4 2.33 mM Tris pH 7.5 50 mM Magnesium chloride hexahydrate MgCl2•6H2O 12.81 mM Methanol 3% mM

Binding Temp: 25 °C°C

Refolding Protocol: 95 °C for 5 min in selection annealing buffer and then immediately placed on ice for 5 min

Specificity: Specific for benzodiazepines

Sequence ():

Length: 95

Molecular Weight: 31350

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Samuelian, John S., and Dana A. Baum. “Identification of DNA aptamers for benzodiazepine detection in synthetic urine.” Analysis & Sensing, vol. 2, no. 6, 10 Aug. 2022, https://doi.org/10.1002/anse.202200044.

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