Aptamer Details

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CArah1-1 (ID# 8730)

Aptamer Chemistry: DNA

Target: Ara h1

Antigen/Target Category: Protein

Affinity (Kd): 200.8 nM (reported value)

Binding Conditions/Buffer: PBSMT: (pH 7.2, 137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.76 mM KH2PO4, 1 mM MgCl2, 0.025% Tween 20) and 20% human serum.

Binding Temp: 25°C

Refolding Protocol: Heat to 95°C for 5 minutes, cool on ice for 15 minutes, incubate with 20% human serum in 100 µL PBSMT at 25°C for 1 hour.

Comments: Circular ssDNA.

Sequence ():

Length: 60

Molecular Weight: 18608.1 Da

Extinction Coefficient: 614300 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yao L, Feng J, Zhou Y, Gao S, Liu S, Qu H, Mao Y, Zheng L. Single-Round Circular Aptamer Discovery Using Bioinspired Magnetosome-Like Magnetic Chain Cross-Linked Graphene Oxide. Research 2024;7:Article 0372. https://doi. org/10.34133/research.0372

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