Aptamer Details

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Protein Kinase R (PKR) Aptamer (ID# 8744)

Aptamer Chemistry: RNA

Target: Protein Kinase R (PKR)

Antigen/Target Category: Protein

IC50: 150 pM (reported value)

Binding Conditions/Buffer: Refolding Buffer: 333 mM HEPES, pH 8.0, 333 mM NaCl, 33 mM MgCl2. After refolding, incubate with 100 mM of 2-methylnicotinic acid imidazolide (NAI) in DMSO for 10 min at 37°C.

Binding Temp: 37°C

Refolding Protocol: 5 min at 65°C in Refolding Buffer, then slowly cool to 23°C.

Comments: Full sequence available soon. Each circular RNA aptamer construct appears to bind 2 PKR protein molecules. 50-150 pmoles of circular RNA aptamer construct appears to have a notable effect on psoriasis plaques in mouse models.

Sequence ():

Length: 364

Molecular Weight: 120120

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Guo, SK., Liu, CX., Xu, YF. et al. Therapeutic application of circular RNA aptamers in a mouse model of psoriasis. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02204-4

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