Aptamer Details

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HSA-6 (ID# 8833)

Aptamer Chemistry: DNA

Target: HSA

Antigen/Target Category: Protein

Affinity (Kd): 20 ± 4 nM (reported value)

Binding Conditions/Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.4

Binding Temp: 23°C

Refolding Protocol: N/A

Comments: Kd varied by method: 20 ± 4nM (Magnetic bead-based qPCR assay), 1.2 ± 0.3nM (ELONA), and 19 ± 3nM (Fluorescence evanescent wave biosensor) Room temperature not specified; presumed to be 23°C

Sequence ():

Length: 60

Molecular Weight: 18576.1

Extinction Coefficient: 599500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Qiao, N., Li, J., Wu, X., Diao, D., Zhao, J., Li, J., … Lou, X. (2019). Speeding up in vitro Discovery of Structure-Switching Aptamers via Magnetic Cross-Linking Precipitation. Analytical Chemistry. doi:10.1021/acs.analchem.9b00081

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