Aptamer Details

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Pb-14S (ID# 8881)

Aptamer Chemistry: DNA

Target: Pb(||)

Antigen/Target Category: Other

Affinity (Kd): 0.76 ± 0.18 µM (reported value)

Binding Conditions/Buffer: SB-buffer (100M Al(NO3)3, 100M Cu(NO3)2, 100M Co(NO3)2, 100M Zn(NO3)2, 100M Ca(NO3)2 and 100M Ba(NO3)2 in SELEX buffer) 200L of SELEX buffer (20 mM Tris-HAc, 10mM NaAc, pH = 7.4) 200L of 100M Pb(NO3)2 in SELEX buffer 200L of SELEX buffer

Binding Temp: 23°C

Refolding Protocol: N/A

Specificity: ~2.1

Sequence ():

Length: 42

Molecular Weight: 12939.4

Extinction Coefficient: 408800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chen Y., Li H., Gao T., Zhang T., Xua L., Wang B., Wang J., & Pei R. (2018). Selection of DNA aptamers for the development of light-up biosensor to detect Pb(II). Sensors and Actuators B: Chemical, 254, 214-221. http://dx.doi.org/10.1016/j.snb.2017.07.068

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