Aptagen, LLC
G-3 aptamer against CCR5 (HIV) (ID# 8990)
RNA
CCR5
Protein
110.7 ± 22.7 nM (reported value)
37°C for 10 min in DPBS (pH 7.0–7.4) and Ca2+ and Mg2+ (1 mM CaCl2, 2.7 mM KCl, 1.47 mM KH2PO4, 1 mM MgCl2, 136.9 mM NaCl, 2.13 mM Na2HPO4) with yeast tRNA at 100 ug/mL).
37°C
RNA in 3 mL of refolding buffer, heated to 65°C for 5 min, slowly cooled to 37°C.
Affinity for CEM-NKR negative cells is >3000 nM.
Binding affinity measured against U373-Magi-CCR5(+) cells. Aptamer G-3 also tested against CEM-NKR-CCR5(+) cells with a Kd of 113.1 ± 28.2 nM.
5'-rUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprAprGprGprAprCprGprAprUprGprCprGprGprGprCprCprUprUprCprGprUprUprUprGprUprUprUprCprGprUprCprCprAprUprCprGprGprGprCprGprAprGprUprCprGprUprCprUprGp-3'
68
21838 g/mole
664000 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhou J, Satheesan S, Li H, Weinberg MS, Morris KV, Burnett JC, Rossi JJ. Cell-specific RNA aptamer against human CCR5 specifically targets HIV-1 susceptible cells and inhibits HIV-1 infectivity. Chem Biol. 2015 Mar 19;22(3):379-90. doi: 10.1016/j.chembiol.2015.01.005. Epub 2015 Mar 5. PMID: 25754473; PMCID: PMC4369413.
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