Aptagen, LLC
H Seq01 (ID# 9187)
DNA
Small Organic
25.3 nM (reported value)
Dulbecco’s phosphate-buffered saline (DPBS), pH 7.4
25 °C
The aptamer was likely refolded by heating and cooling, though the exact protocol for H Seq01 is not specified in the paper. For similar aptamers in the study, the general refolding protocol involved: Heating at 95°C for 5 minutes to denature secondary structures. Cooling to room temperature for 30 minutes to allow proper refolding.
The study did not provide specific cross-reactivity data for H Seq01. However, the overall screening process involved negative and counter-selection steps to remove nonspecific binders, suggesting that H Seq01 has some level of specificity for ST. The primary aptamer, H Seq02, showed excellent specificity for ST, with minimal cross-reactivity to other mycotoxins.
H Seq01 was identified through high-throughput sequencing after 16 rounds of capture-SELEX screening.
It was one of the candidate sequences selected for further analysis based on its abundance and secondary structure.
The study primarily focused on H Seq02, which showed the highest affinity and specificity for ST.
H Seq01 was predicted to form stable secondary structures, which may contribute to its binding properties.
5'-dApdCpdTpdCpdCpdApdCpdApdGpdCpdApdTpdApdGpdGpdTpdTpdApdTpdApdCpdCpdGpdApdApdCpdApdGpdTpdApdApdTpdCpdCpdGpdTpdApdTpdTpdCp-3'
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
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