Aptamer Details

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API7-4 (ID# 9205)

Aptamer Chemistry: DNA

Antigen/Target Category: Small Organic

Affinity (Kd): < 10 nM (reported value)

Binding Conditions/Buffer: Binding buffer: PBST (Phosphate Buffered Saline with Tween 20) + 5 mM Mg²⁺

Binding Temp: 37°C

Refolding Protocol: Not explicitly mentioned in the paper. However, the aptamers were likely refolded by heating and cooling, a common practice in aptamer studies, though the specific program is not detailed.

Comments: Specifically binds to HuNoV GII.4 VLP. Does not react with other diarrhea-causing pathogens or unrelated proteins, such as: E. coli lysate Caco2 cell lysate EV71 (Enterovirus 71) Coronavirus S protein Astrovirus Confirmed by dot blotting and ELASA (Enzyme-Linked Aptamer Sorbent Assay).

Sequence ():

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Prasad BVV, Hardy ME, Dokland T, Bella J, Rossmann MG, Estes MK. X-ray crystallographic structure of the Norwalk virus capsid. Science. 1999;286(5438):287–290. doi:10.1126/science.286.5438.287

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