Aptagen, LLC
DNA
Ochratoxin A (OTA)
Small Organic
96 nM (reported value)
Binding Buffer: PBS 1X, 1 mM MgCl2, 0.01% Tween 20, pH 7.4.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This aptamer showed minimal cross-reactivity with ochratoxin B and phenylalanine.
The limit of detection for the enzyme-linked assay was 1 nM with a linear working range from 1-80 nM.
Ochratoxin A (H12) (ID# 392)
5'dGpdGpdGpdApdGpdGpdApdCpdGpdAp dApdGpdCpdGpdGpdApdApdCpdCpdGp dGpdGpdTpdGpdTpdGpdGpdGpdTpdGp dCpdCpdTpdTpdGpdApdTpdCpdCpdAp dGpdGpdGpdApdGpdTpdCpdTpdCpdApdGpdApdApdGpdApdCpdApdCpdGpdCp dCpdCpdGpdApdCpdAp3'
67
20569.34 g/mole
649700 L/(mole·cm)
63.64%
1.54
31.66
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
L Barthelmebs et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine. Food Control 22(2011): 737-743
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