Aptagen, LLC
RNA
L-Isoleucine
Small Organic
200 µM (reported value)
Selection Buffer (10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl) Reaction Buffer (50 mM sodium borate (pH 8.0), 42 mM KCl, 7.5 mM MgCl2, and 0.4 mM ZnCl2)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
L-Isoleucine (IL 42-32b) (ID# 112)
5'rGprGprUprCprUprUprAprCprGprUprCprGprUprUprCprGprCprGprAprCprUprAprUprUprGprGprGprAprGprAprCprCp3'
0
10233.16 g/mole
310800 L/(mole·cm)
56.25%
3.22
32.93
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Majerfeld and Yarus. "Isoleucine:RNA sites with associated coding sequence." RNA, 4 (1998): 471-478.
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