Aptagen, LLC
DNA
Taq DNA polymerase
Protein
36 pM (reported value)
Binding Buffer (50 mM KCl, 2.5 mM MgCl2, 10 mM Tris-HCl (pH 8.3))
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Has affinity for Tth DNA polymerase with Kd of 10 nM.
Taq DNA polymerase (TQ21) (ID# 71)
5'dTpdTpdCpdTpdCpdGpdGpdTpdTpdGpdGpdTpdCpdTpdCpdTpdGpdGpdCpdGpdGpdApdGpdCpdGpdApdTpdCpdApdTpdCpdTpdCpdApdGpdApdGpdCpdApdTpdTpdCpdTpdTpdApdGpdCpdGpdTpdTpdTpdTpdGpdTpdTpdCpdTpdTpdGpdTpdGpdTpdApdTpdGpdApdTpdTpdCpdGpdCpdTpdTpdTpdTpdCpdCpdCp3'
78
23951.56 g/mole
703200 L/(mole·cm)
47.44%
1.42
34.06
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Sumedha and Dang. "Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR." Journal of Molecular Biology, 264 (1996): 268-78.
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