Aptamer Details

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1.5 M Short Anti-Heparanase Aptamer (ID# 7956)

Aptamer Chemistry: DNA

Target: Heparanase

Antigen/Target Category: Protein

Affinity (Kd): n/a nM (reported value)

Binding Conditions/Buffer: Cell Culture Medium: 50% DMEM, 50% Hamƒ??s F-12 Cell culture medium supplemented with: 50 mg/ml ascorbic acid, 250 ng/ml amphotericin B, 5mg/ml insulin (bovine pancreas), 0.4 ng/ml hydrocortisone and 10% heat-inactivated fetal bovine serum.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 30

Molecular Weight: 9254.11

Extinction Coefficient: 298000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Simmons SC, Jamsa H, Silva D, Cortez CM, McKenzie EA, et al. (2014) Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer. PLoS ONE 9(10): e96846. doi:10.1371/journal.pone.0096846

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