Aptamer Details

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A15 (ID# 7913)

Aptamer Chemistry: DNA

Target: Staphylococcus aureus enterotoxin A (SEA)

Antigen/Target Category: Protein

Affinity (Kd): 48.57 nM (reported value)

Binding Conditions/Buffer: 20 mM Tris, 100 mM NaCl, 5 mM KCl, 1 mM CaCl2 and 1 mM MgCl2 ƒ?› 6H2O, pH 7.4

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: ssDNA aptamers specific for SEA were selected using a 12-round SELEX procedure. A15 was used successfully in fluorescent bioassay to detect SEA in the food sample with a detection limit of 8.7 x 10^(-3) ug/mL.

Sequence ():

Length: 80

Molecular Weight: 24604.02

Extinction Coefficient: 768000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Huang, Y. et al. "Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A." Anal. Methods, 2014, 6, 690-697.

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