Aptamer Details

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Glucan-Binding Protein C (GbpC) A96 (ID# 8820)

Aptamer Chemistry: DNA

Target: S. mutans Glucan-Binding Protein C

Antigen/Target Category: Protein

Affinity (Kd): 440.5 nM (reported value)

Binding Conditions/Buffer: Artificial Saliva Buffer (pH 7.0): 15.06 mM NaCl, 1.54 mM KH2PO4, 2.46 mM K2HPO4, 13.95 mM KCl, 1.96 KSCN, 1.01 CaClH2O, 0.18 mM Cl2H14MgO7, 2.06 mM NH4Cl, 5.00 mM NaHCO3, 2.00 mM Urea, 1.17 mM ProClin(TM) 300 (preservative)

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This article is a preprint and has not been certified by peer review

Sequence ():

Length: 76

Molecular Weight: 23024.9 g/mole

Extinction Coefficient: 690100 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Selection of streptococcal glucan-binding protein C specific DNA aptamers to inhibit biofilm formation Akos Harkai, Yoon Kee Beck, Anna Tory, Tamas Meszaros bioRxiv 2024.08.23.608024; doi: https://doi.org/10.1101/2024.08.23.608024

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