Aptamer Details

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Adenosine Triphosphate (1-1MIN) (ID# 7510)

Aptamer Chemistry: RNA

Target: Adenosine Triphosphate / ATP

Antigen/Target Category: Small Organic

Affinity (Kd): 4.8 µM (reported value)

Binding Conditions/Buffer: 300 mM NaCl, 10 mM MgCl₂ OR 30 mM MgCl₂, 0.1 mM EDTA, and 25 mM Tris (pH 7.8)

Binding Temp: N/A°C

Refolding Protocol: N/A

Specificity: Approximately 150-fold difference between ATP and AMP at 10 mM MgCl₂.

Approximately 1100-fold difference between ATP and AMP at 30 mM MgCl₂.

Comments: Kd value listed is for 30 mM MgCl₂. Kd value at 10 mM MgCl₂ was 11 µM.

Sequence ():

Length: 57

Molecular Weight: 18459.16

Extinction Coefficient: 571900

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

P Sazani, R Larralde, and J Szostak.(2004) "A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP." J Am Chem Soc. 126: 8370-8371.

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