Aptamer Details

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AHD50-1 against 1-aminohydantoin (ID# 8762)

Aptamer Chemistry: DNA

Target: 1-aminohydantoin (AHD)

Antigen/Target Category: Small Organic

Affinity (Kd): 86 nM (reported value)

Binding Conditions/Buffer: Binding buffer: pH 7.4. 100mM NaCl, 2mM MgCl2, 20mM Tris-HCl, 1 mM CaCl2, 5mM KCl. 25°C in the dark for 2 hours.

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Singled stranded, truncated.

Sequence ():

Length: 27 bases

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zheng, X., Jiang, S., Ren, Y., Wang, S., Xie, Y., & Le, T. (2024). High-efficient selection of aptamers by magnetic cross-linking precipitation and development of aptasensor for 1-aminohydantoin detection. LWT - Food Science and Technology, 199. https://doi.org/10.1016/j.lwt.2024.116128

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