Aptamer Details

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Anti-FLAg M2 Antibody Aptamer (ABA) (ID# 7715)

Aptamer Chemistry: DNA

Target: Anti-FLAG M2 Antibody

Antigen/Target Category: Protein

Affinity (Kd): 80 nM (reported value)

Binding Conditions/Buffer: 4% glycerol, 1 mM MgCL₂, 1 mM DTT, 50 mM NaCl, 10 mM Tris-HCl, pH 7.5, r.t.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 30

Molecular Weight: 9160.02

Extinction Coefficient: 274800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

A Lakamp and M Ouellette. A ssDNA aptamer that blocks the function of the anti-FLAG M2 antibody. J. Nucleic Acids 2011: doi:10.4061/2011/720798

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