Aptamer Details

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Anti-His Tag (6H7) (ID# 7564)

Aptamer Chemistry: DNA

Target: 6X His Tag

Antigen/Target Category: Protein

Affinity (Kd): 4.6 nM (reported value)

Binding Conditions/Buffer: Selection Buffer (50mM K2HPO4, 150mM NaCl, 0.05% Tween 20 (pH 7.5))

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 40

Molecular Weight: 12488.1

Extinction Coefficient: 370000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kokpinar, et al. "Aptamer-Based Downstream Processing of His-Tagged Proteins Utilizing Magnetic Beads." Biotechnology and Bioengineering, 108(2011):2371-2379.

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