Aptagen, LLC
Anti-HSA Structure-switching Aptamer (ID# 8060)
DNA
HSA (Human Serum Albumin)
Protein
20 ± 4 nM (reported value)
Binding buffer for anti-HSA aptamer selection: 50 mM HEPES, 100 mM NaCl, 1 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.4.
23°C
95°C for 10 minutes, slow cooling to room temperature
Demonstrated specificity to HSA over streptavidin, short peptides, and BSA (bovine serum albumin).
5’-dCpdGpdTpdApdCpdCpdGpdGpdCpdCpdApdGpdTpdGpdApdTpdTpdApdCpdGpdApdCpdGpdApdGpdApdCpdGpdApdGpdCpdTpdTpdApdTpdGpdCpdGpdTpdApdTpdTpdGpdApdTpdGpdCpdCpdTpdApdApdCpdTpdApdTpdCpdTpdApdCpdAp
-3'
60
18459
582100
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Qiao, N., Li, J., Wu, X., Diao, D., Zhao, J., Li, J., … Lou, X. (2019). Speeding up in vitro Discovery of Structure-Switching Aptamers via Magnetic Cross-Linking Precipitation. Analytical Chemistry. doi:10.1021/acs.analchem.9b00081
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