Aptamer Details

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Anti Lung Carcinoma ( EJ4) (ID# 7871)

Aptamer Chemistry: DNA

Target: Lung Adenocarcinoma (H23)

Antigen/Target Category: Cells

Affinity (Kd): 60 nM (reported value)

Binding Conditions/Buffer: 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes

Binding Temp: 4°C

Refolding Protocol: denature by heating at 95C for 5 minutes, followed by rapid cooling on ice

Specificity: HBE 135-E6/E7 (normal epithelial lung) cells used as counter targets

Comments: Reference did not list what primer sequences they used. The Author only mentioned that the primers were designed using IDTƒ??s oligo analyzer and met the following characteristics: a minimum hairpin structure, similar melting temperatures and minimal base pairing.

Sequence ():

Length: 41

Molecular Weight: 12668.28

Extinction Coefficient: 402800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jimenez et al. Generation of Lung Adenocarcinoma DNA Aptamers for Cancer Studies (PLOSone)

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