Aptamer Details

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Anti-NF-kB p65 (D1) (ID# 7754)

Aptamer Chemistry: RNA

Target: Anti-NF-kB p65

Antigen/Target Category: Protein

Affinity (Kd): 149 nM (reported value)

Binding Conditions/Buffer: Selection buffer: 10mM HEPES at pH 7.5, 100mM NaCl, 1mM DTT

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds to both p65 and p50, with kd of 149nM and 190nM respectively.

Sequence ():

Length: 85

Molecular Weight: 27468.57

Extinction Coefficient: 858400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wurster, S. E, and Maher, L. J. "Selection and characterization of anti-NF-kB p65 aptamers." RNA, 14(2008): 1037-1047.

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