Aptamer Details

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AP-1 cJun/cJun Aptamer-19 (ID# 7940)

Aptamer Chemistry: DNA

Target: Activator Protein-1 Family cJun/cJun Homodimer

Antigen/Target Category: Protein

Affinity (Kd): 0.5 nM (reported value)

Binding Conditions/Buffer: binding buffer (20 mM Tris (pH 7.9), 0.1 mM EDTA, 10% glycerol, 1 mM DTT, 150 mM KCl, 5 mM MgCl2, 0.04% NP-40)

Binding Temp: 24°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: kD for counter-target cJun/cFos = 85 nM

Sequence ():

Length: 78

Molecular Weight: 24206.74

Extinction Coefficient: 762100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Walters RD, McSwiggen DT, Goodrich JA, Kugel JF (2014) Selection and Characterization of a DNA Aptamer That Can Discriminate between cJun/cJun and cJun/cFos. PLoS ONE 9(6): e101015. doi:10.1371/journal.pone.0101015

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