Aptamer Details

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Azo-1 (ID# 8135)

Aptamer Chemistry: DNA

Target: Human Thrombin

Antigen/Target Category: Protein

Affinity (Kd): Not given. nM (reported value)

Binding Conditions/Buffer: 20 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 10 mM NaCl, and 0.05%Tween-20.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Each U in the sequence is a dU^Az residue. These are azobenzene-modified deoxyuridine.

Sequence ():

Length: 70

Molecular Weight: 21442.7

Extinction Coefficient: 658400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Morihiro, K.; Hasegawa, O.; Kasahara, Y.; Mori, S.; Kasai, T.; Kuwahara, M.; Obika, S. Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method. Bioorg. Med. Chem. Lett. 2021 Jan 1; 31: 127607. DOI: 10.1016/j.bmcl.2020.127607.

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