Aptamer Details

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B1-4 (ID# 7903)

Aptamer Chemistry: DNA

Target: IFN-Gamma

Antigen/Target Category: Protein

Affinity (Kd): 74.5 nM (reported value)

Binding Conditions/Buffer: 200 uL (PBS), 0.05 pM Aptamer

Binding Temp: 24°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Tested against BSA, Trypsin, IgG and Albumin, displays a high degree of specificity

Sequence ():

Length: 59

Molecular Weight: 18001.76

Extinction Coefficient: 585100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Cao B, Hu Y, Duan J, Ma J, Xu D, et al. (2014) Selection of a Novel DNA Aptamer for Assay of Intracellular Interferon-Gamma. PLoS ONE 9(5): e98214. doi:10.1371/journal.pone.0098214

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