Aptamer Details

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Basic Fibroblast Growth Factor (26A-t) (ID# 7540)

Aptamer Chemistry: RNA

Target: Basic Fibroblast Growth Factor (bFGF)

Antigen/Target Category: Protein

Affinity (Kd): 190 pM (reported value)

Binding Conditions/Buffer: Selection Buffer (1X PBS = 10.1 mM Na2HPO4/1.8 mMKH2PO4/137 mM NaCl/2.7 mM KCl, pH 7.4)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Heat-denatured bFGF (90C for 15 min) binds to aptamer with Kd > 1uM. This is a biphasic binding with Kd 0.19+-0.02 nM.

Sequence ():

Length: 34

Molecular Weight: 10943.71

Extinction Coefficient: 340500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jellinek, D., et al. "High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding." Proceedings of the National Academy of Sciences of the United States of America, 90(1993): 11227ƒ??11231.

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