Aptagen, LLC
CAP/CAM Aptamer (ID# 7981)
RNA
Chloramphenicol
Other
7.7 µM (reported value)
100 mM NaCl, 20 mM Trisƒ??HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
AptaReport Article Reference: Luan Q., Xi Y., et al. (2017) A facile colorimetric aptamer assay for small molecule detection in food based on a magnetic single-stranded DNA binding protein-linked composite probe. Sensors and Actuators B: Chemical 239: 979-978.
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdCpdTpdTpdCpdApdGpdTpdGpdApdGpdTpdTpdGpdTpdCpdCpdCpdApdCpdGpdGpdTpdCpdGpdGpdCpdGpdApdGpdTpdCpdGpdGpdTpdGpdGpdTpdApdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdCpdGpdTpdGpdApdAp-3'
81
25106.28
781800
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Mehta J, Van Dorst B, Rouah-Martin E, et al. (2010) In vitro selection and characterication of DNA aptamers recognizing chloramphenicol. Journal of Biotechnology 155: 361-369.
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