Aptamer Details

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CoV RBD Protein (Linear Aptamer) (ID# 8431)

Aptamer Chemistry: DNA

Target: CoV Spike Protein

Antigen/Target Category: Protein

Affinity (Kd): 10.9 µM (reported value)

Binding Conditions/Buffer: Buffer: 1 ml of 1× SELEX buffer (20 mM Tris-HCl (pH:7.4), 2 mM CaCl2, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2).

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 50

Molecular Weight: 16500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Simple in-vitro single stranded linear and circular DNA preparation and validation via SELEX using phosphor-derived modifications Seyed Vahid Hamidi, Jonathan Perreault bioRxiv 2023.02.11.528153; doi: https://doi.org/10.1101/2023.02.11.528153

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