Aptamer Details

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DB99 (Clone A) (ID# 8196)

Aptamer Chemistry: RNA

Target: GD2

Antigen/Target Category: Protein

Affinity (Kd): 21.21 nM (reported value)

Binding Conditions/Buffer: 1X PBS (pH 7.4) Selections carried out in vivo in six- to eight-week-old female immunodeficient BALB/c mice (CAnN. Cg-Foxn1nu/Crl).

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: No binding to A431, A172, or Molm-13 cells - GD2-deficient cell lines.

Comments: The sequence for Clone B was identified, but that construct was not studied.

Sequence ():

Length: 66

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zhang, L., Wang, M., Zhu, Z., Chen, S., Wu, H., Yang, Y., Che, F., Li, Q., & Li, H. (2021). A GD2-aptamer-mediated, self-assembling nanomedicine for targeted multiple treatments in neuroblastoma theranostics. Molecular therapy. Nucleic acids, 26, 732–748. https://doi.org/10.1016/j.omtn.2021.08.021

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