Aptamer Details

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Dopamine (dopa2/c.4) (ID# 7766)

Aptamer Chemistry: RNA

Target: Dopamine

Antigen/Target Category: Small Organic

LOD: 1 pM (reported value)

Binding Conditions/Buffer: Assay Buffer: 10 mM PBS, pH 7.2, 5mM MgCl₂

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Cross-reactivity was performed on 3-methoxytyramine, epinephrine, homovanillic acid, 3,4-dihydroxyphenylacetic acid (cross-reactivity less than 1% for these reactants) and norepinephrine (3.68%).

Sequence ():

Length: 67

Molecular Weight: 21676.14

Extinction Coefficient: 652400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973.
Enzyme-linked assay from H Park and I R Paeng. "Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum." Analytica Chimica Acta, 685 (2011): 65-73.

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