Aptamer Details

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E. Coli 5S RNA (Helix 89 RNA) (ID# 7516)

Aptamer Chemistry: RNA

Target: E. Coli 5S RNA

Antigen/Target Category: Small Organic

Affinity (Kd): 3 µM (reported value)

Binding Conditions/Buffer: Binding buffer: 30 mM Trisƒ??acetate(pH 7.5), 60 mM magnesium acetate, 120 mM potassium acetate, and 120 mM ammonium acetate.

Binding Temp: RT°C

Refolding Protocol: in Binding buffer, heat to 55° C for 15 minutes and slowly let cool to RT.

Sequence ():

Length: 37

Molecular Weight: 11910.22

Extinction Coefficient: 366600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ko, J. et al. "Identification of a structural motif of 23S rRNA interacting with 5S rRNA." FEBS Letters, 508 (2001): 300-304.

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