Aptamer Details

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Endothelial Integrin Alpha-V Beta-3 (Clone 17.16) (ID# 7613)

Aptamer Chemistry: RNA

Target: Alpha-V Beta-3

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Washed with PBS and 0.9 mM CaCl₂, 0.5 mM MgCl₂, 2.67 mM KCl, 1.47 mM KH₂PO₄, 135 mM NaCl, and 8 mM Na₂.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: See also PF377 P-Selectin Aptamer for an aptamer that also decreases sickled RBC binding to HUVECs.

Sequence ():

Length: 37

Molecular Weight: 11855.18

Extinction Coefficient: 364200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Brunette et al. "RNA Aptamer Therapy for Vaso-Occlusion in Sickle Cell Disease." Nucleic Acids Therapeutics, 21(2011): 275-283. Mi et al. "Targeted inhibition of av??3 integrin with an RNA aptamer impairs endothelial cell growth and survival." Biochemical and Biophysical Research Communications, 338(2005): 956-963.

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