Aptagen, LLC
Enterotoxigenic Escherichia coli (E. coli) fimbriae protein K88 (clone 37) (ID# 7924)
DNA
K88
Protein
25 nM (reported value)
20 mmol/L HEPES (pH 7.35), 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KCl
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding protocol:
The ssDNA pools (800 pmol for initial round) were heated at 95 °C
for 5 min in a binding buffer (SHCMK) containing 20 mmol/L
HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid),
pH 7.35; 1 mmol/L MgCl2; 1 mmol/L CaCl2; and
120 mmol/L KCl; and were then cooled to room temperature.
Sequence selected from library design: 5′-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3′. Full aptamer sequence should probably include library primers, but this was not specified in the reference.
5'-dGpdApdGpdApdCpdCpdGpdTpdApdCpdCpdApdTpdCpdTpdGpdTpdTpdCpdGpdTpdGpdGpdApdApdGpdCpdGpdCpdTpdTpdTpdGpdCpdTpdCpdGpdTpdCpdCpdApdTpdTpdApdGpdCpdCpdTpdTpdGpdTpdGpdCpdTpdCpdGpdTpdGpdCp-3'
59
18431.95
545200
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Li, H. et al. Aptamer selection for the detection of Escherichia coli K88. 2011. Canadian Journal of Microbiology 57.6: 453-9.
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