Aptamer Details

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EP23 (ID# 8104)

Aptamer Chemistry: RNA

Target: Epithelial cellular activating molecule (EpCAM)

Antigen/Target Category: Cells

Affinity (Kd): 39.89 nM (reported value)

Binding Conditions/Buffer: PBS containing 5 mM MgCl2

Binding Temp: 25 and 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 19

Molecular Weight: 5631.6

Extinction Coefficient: 165300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Bell DR, Weber JK, Yin W, Huynh T, Duan W, Zhou R. In silico design and validation of high-affinity RNA aptamers targeting epithelial cellular adhesion molecule dimers. Proc Natl Acad Sci U S A. 2020 Apr 14;117(15):8486-8493. doi: 10.1073/pnas.1913242117. Epub 2020 Mar 31. Erratum in: Proc Natl Acad Sci U S A. 2021 Feb 16;118(7): PMID: 32234785; PMCID: PMC7165443.

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