Aptamer Details

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EphA2 Receptor (SWL peptide) (ID# 7627)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: EphA2 Receptor

Antigen/Target Category: Tissue

Affinity (Kd): 678 nM (reported value)

Binding Conditions/Buffer: Blocked with 0.5% BSA in TBS and washed with binding buffer (TBS, 1 mM CaCl2, 0.1% Tween 20).

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: See also the YSA peptide targeting this protein.

Sequence ():

Length: 12

Molecular Weight: 1505.73

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Koolpe et al. "An Ephrin Mimetic Peptide That Selectively Targets the EphA2 Receptor." J. Biol. Chem., 49(2002): 46974 ƒ??4697.

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